The effects of trace elements on the lipid productivity and fatty acid composition of Nannochloropis oculata (N. oculata)were studied. The results showed that trace elements had a strong influence on not only the lipid productivity but also the fatty acid composition. The addition of Fe 3+, Zn 2+, Mn 2+, Mo 6+, and EDTA and the deletion of Cu 2+and Co 2+can increase the lipid productivity. The optimum concentrations of the trace elements in the culture medium are 6 times of Fe 3+and EDTA, the same concentration of Zn 2+, Mn 2+, and Mo 6+as the control group, but the optimum medium has no Cu 2+or Co 2+. Fe 3+, Zn 2+, Mn 2+, Mo 6+, and EDTA are indispensable during the EPA formation of N. oculata. The addition of Fe 3+, Zn 2+, Mn 2+, Mo 6+, and EDTA can strongly increase the content of EPA in the lipid of N. oculata, but the concentration of the trace elements had little influence on the level of EPA.
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