Concurrent NP-HPTLC Determination of Shikonin and β,β-Dimethylacryl Shikonin in Arnebia benthamii. – ScienceOpen
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      Concurrent NP-HPTLC Determination of Shikonin and β,β-Dimethylacryl Shikonin in Arnebia benthamii.

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          Abstract

          Naphthoquinones are important class of molecules found as a natural red color pigments in roots of Arnebia benthamii (Wall. ex G. Don) L M. Johnston. The aim of present investigation is to develop and validates a simple, cost-effective and reliable method for quantification of these compounds. Therefore, a normal phase-high performance thin-layer chromatography (NP-HPTLC) method for concurrent determination of shikonin and β,β-dimethylacryl shikonin in A. benthamii was established. Method development of naphthoquinones in the methanol extract was done using hexane-ethyl acetate-methanol (40:7.5:2.5, v/v/v) solvent system at 520 nm. The developed method showed good band separation for shikonin (Rf, 0.37) and β,β-dimethylacryl shikonin (Rf, 0.58). The linearity ranged between 100 and 8,000 ng spot(-1) with an average recovery of >97% in both cases. The results showed reproducible intraday and interday precision (<2.0% RSD) in quantification of naphthoquinones. The limits of detection are 12.96 and 14.65 ng spot(-1) while the limits of quantification are 39.27 and 44.39 ng spot(-1) for shikonin and β,β-dimethylacryl shikonin, respectively. The developed method is reliable, fast, easy to follow and economic in concurrent assessment of shikonin and β,β-dimethylacryl shikonin in A. benthamii root samples. In addition, it seems to be first report for identification and quantification of β,β-dimethylacryl shikonin from the A. benthamii.

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          Author and article information

          Journal
          J Chromatogr Sci
          Journal of chromatographic science
          Oxford University Press (OUP)
          1945-239X
          0021-9665
          Sep 2016
          : 54
          : 8
          Affiliations
          [1 ] Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India.
          [2 ] Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India.
          [3 ] Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India sharmadinesh82@gmail.com dineshkumar@ihbt.res.in.
          [4 ] Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India.
          Article
          bmw096
          10.1093/chromsci/bmw096
          27278171
          3273b86b-1007-4263-b4cd-4df9c8be1948
          © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
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