Heatwaves cause fluctuations in wMel Wolbachia densities and frequencies in Aedes aegypti

Abstract

Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia are being released into natural mosquito populations in the tropics as a way of reducing dengue transmission. High temperatures adversely affect wMel, reducing Wolbachia density and cytoplasmic incompatibility in some larval habitats that experience large temperature fluctuations. We monitored the impact of a 43.6°C heatwave on the wMel infection in a natural population in Cairns, Australia, where wMel was first released in 2011 and has persisted at a high frequency. Wolbachia infection frequencies in the month following the heatwave were reduced to 83% in larvae sampled directly from field habitats and 88% in eggs collected from ovitraps, but recovered to be near 100% four months later. Effects of the heatwave on wMel appeared to be stage-specific and delayed, with reduced frequencies and densities in field-collected larvae and adults reared from ovitraps but higher frequencies in field-collected adults. Laboratory experiments showed that the effects of heatwaves on cytoplasmic incompatibility and density are life stage-specific, with first instar larvae being the most vulnerable to temperature effects. Our results indicate that heatwaves in wMel-infected populations will have only temporary effects on Wolbachia frequencies and density once the infection has established in the population. Our results are relevant to ongoing releases of wMel-infected Ae. aegypti in several tropical countries.

Author summary

Mosquitoes infected with Wolbachia bacteria are being released in the tropics to replace natural mosquito populations and suppress dengue transmission. Aedes aegypti mosquitoes with the wMel strain of Wolbachia were first released in Cairns, Australia in 2011 and releases were then expanded to the entire city and surrounding suburbs. Today, wMel is at a high frequency within the Ae. aegypti population and local dengue transmission in Cairns has declined to nearly zero. Wolbachia infections are vulnerable to high temperatures and the ability of wMel to persist in populations and block dengue may be constrained by climate. Cairns experienced a record heatwave of 43.6°C in November 2018 and we wanted to see whether this affected the wMel-infected Ae. aegypti population. Our results show that the frequency and density of wMel declined after the heatwave, with effects depending on the mosquito life stage tested. When we monitored the population again in April 2019, wMel had returned to a high frequency. We suggest that heatwaves of the magnitude experienced in Cairns will not have long-term impacts on the wMel infection but may affect invasion during releases or interfere with dengue blockage. Heatwaves may affect interventions with wMel-infected Ae. aegypti that are being deployed in several countries. Effects may depend on the proportion of larval habitats that are protected from extreme temperature fluctuations.