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      The anti-allergic potential of stingless bee honey from different botanical sources via modulation of mast cell degranulation

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          Abstract

          Background

          Allergy is an inflammatory disorder affecting around 20% of the global population. The adverse effects of current conventional treatments give rise to the increased popularity of using natural food products as complementary and alternative medicine against allergic diseases. Stingless bee honey, commonly known as Kelulut honey (KH) in Malaysia, has been used locally as a traditional remedy to relieve cough and asthma. This study evaluated the anti-allergic potential of KH collected from four different botanical sources on phorbol ester 12-myristate-3-acetate and calcium ionophore-activated human mast cells.

          Methods

          The present study examined the inhibitory effects of all collected honey on the release of selected inflammatory mediators, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-4, IL-6, IL-8, histamine, and β-hexosaminidase in an activated HMC. Besides that, all honey's total phenolic content (TPC) was also examined, followed by using liquid chromatography with tandem mass spectrometry (LC–MS/MS) to identify the phytochemicals in the honey. Further examination of the identified phytochemicals on their potential interaction with selected signaling molecules in an activated mast cell was conducted using computational methods.

          Results

          The results indicated that there were significant inhibitory effects on all selected inflammatory mediators’ release by KH sourced from bamboo (BH) and rubber tree (RH) at 0.5% and 1%, but not KH sourced from mango (AH) and noni (EH). BH and RH were found to have higher TPC values and were rich in their phytochemical profiles based on the LC–MS/MS results. Computational studies were employed to determine the possible molecular target of KH through molecular docking using HADDOCK and PRODIGY web servers.

          Conclusions

          In short, the results indicated that KH possesses anti-allergic effects towards an activated HMC, possibly by targeting downstream MAPKs. However, their anti-allergic effects may vary according to their botanical sources. Nevertheless, the present study has provided insight into the potential application of stingless bee honey as a complementary and alternative medicine to treat various allergic diseases.

          Supplementary Information

          The online version contains supplementary material available at 10.1186/s12906-023-04129-y.

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          Most cited references67

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          The HADDOCK2.2 Web Server: User-Friendly Integrative Modeling of Biomolecular Complexes.

          The prediction of the quaternary structure of biomolecular macromolecules is of paramount importance for fundamental understanding of cellular processes and drug design. In the era of integrative structural biology, one way of increasing the accuracy of modeling methods used to predict the structure of biomolecular complexes is to include as much experimental or predictive information as possible in the process. This has been at the core of our information-driven docking approach HADDOCK. We present here the updated version 2.2 of the HADDOCK portal, which offers new features such as support for mixed molecule types, additional experimental restraints and improved protocols, all of this in a user-friendly interface. With well over 6000 registered users and 108,000 jobs served, an increasing fraction of which on grid resources, we hope that this timely upgrade will help the community to solve important biological questions and further advance the field. The HADDOCK2.2 Web server is freely accessible to non-profit users at http://haddock.science.uu.nl/services/HADDOCK2.2.
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            PLIP 2021: expanding the scope of the protein–ligand interaction profiler to DNA and RNA

            With the growth of protein structure data, the analysis of molecular interactions between ligands and their target molecules is gaining importance. PLIP, the protein–ligand interaction profiler, detects and visualises these interactions and provides data in formats suitable for further processing. PLIP has proven very successful in applications ranging from the characterisation of docking experiments to the assessment of novel ligand–protein complexes. Besides ligand–protein interactions, interactions with DNA and RNA play a vital role in many applications, such as drugs targeting DNA or RNA-binding proteins. To date, over 7% of all 3D structures in the Protein Data Bank include DNA or RNA. Therefore, we extended PLIP to encompass these important molecules. We demonstrate the power of this extension with examples of a cancer drug binding to a DNA target, and an RNA–protein complex central to a neurological disease. PLIP is available online at https://plip-tool.biotec.tu-dresden.de and as open source code. So far, the engine has served over a million queries and the source code has been downloaded several thousand times. Graphical Abstract The Protein-Ligand Interaction Profiler. PLIP detects molecular interactions in 3D structures including DNA and RNA.
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              Mast Cell: A Multi-Functional Master Cell

              Mast cells are immune cells of the myeloid lineage and are present in connective tissues throughout the body. The activation and degranulation of mast cells significantly modulates many aspects of physiological and pathological conditions in various settings. With respect to normal physiological functions, mast cells are known to regulate vasodilation, vascular homeostasis, innate and adaptive immune responses, angiogenesis, and venom detoxification. On the other hand, mast cells have also been implicated in the pathophysiology of many diseases, including allergy, asthma, anaphylaxis, gastrointestinal disorders, many types of malignancies, and cardiovascular diseases. This review summarizes the current understanding of the role of mast cells in many pathophysiological conditions.
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                Author and article information

                Contributors
                chauling@upm.edu.my
                Tan.jiwei@monash.edu
                Journal
                BMC Complement Med Ther
                BMC Complement Med Ther
                BMC Complementary Medicine and Therapies
                BioMed Central (London )
                2662-7671
                4 September 2023
                4 September 2023
                2023
                : 23
                : 307
                Affiliations
                [1 ]GRID grid.440425.3, ISNI 0000 0004 1798 0746, School of Science, , Monash University Malaysia, ; Jalan Lagoon Selatan, Bandar Sunway, Subang Jaya, 47500 Selangor Malaysia
                [2 ]GRID grid.1002.3, ISNI 0000 0004 1936 7857, Australian Regenerative Medicine Institute, , Monash University, ; Clayton, 3800 VIC Australia
                [3 ]GRID grid.11875.3a, ISNI 0000 0001 2294 3534, Institute for Research in Molecular Medicine (INFORMM), , Universiti Sains Malaysia, ; Pulau Pinang, 11800 Malaysia
                [4 ]GRID grid.11142.37, ISNI 0000 0001 2231 800X, Department of Biomedical Science, Faculty of Medicine and Health Sciences, , Universiti Putra Malaysia, ; Serdang, 43400 Selangor Malaysia
                [5 ]GRID grid.11142.37, ISNI 0000 0001 2231 800X, Natural Medicines and Products Research Laboratory, Institute of Bioscience, , Universiti Putra Malaysia, ; Serdang, 43400 Selangor Malaysia
                Article
                4129
                10.1186/s12906-023-04129-y
                10476411
                37667314
                be5bfe88-1927-4bde-86a0-f8577a46b3f1
                © BioMed Central Ltd., part of Springer Nature 2023

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

                History
                : 15 March 2023
                : 16 August 2023
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/501100003093, Ministry of Higher Education, Malaysia;
                Award ID: FRGS/1/2018/SKK06/MUSM/03/1
                Award ID: FRGS/1/2018/SKK06/MUSM/03/1
                Award Recipient :
                Funded by: FundRef http://dx.doi.org/10.13039/501100010699, Monash University Malaysia;
                Award ID: STG-000170
                Award Recipient :
                Categories
                Research
                Custom metadata
                © BioMed Central Ltd., part of Springer Nature 2023

                stingless bee honey,kelulut honey,botanical sources,anti-allergic,anti-inflammation,human mast cells,pmaci,polyphenols,in silico

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