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      In-vitro meat: a promising solution for sustainability of meat sector

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          Abstract

          The in-vitro meat is a novel concept in food biotechnology comprising field of tissue engineering and cellular agriculture. It involves production of edible biomass by in-vitro culture of stem cells harvested from the muscle of live animals by self-organizing or scaffolding methodology. It is considered as efficient, environmental friendly, better ensuring public safety and nutritional security, as well as ethical way of producing meat. Source of stem cells, media ingredients, supply of large size bioreactors, skilled manpower, sanitary requirements, production of products with similar sensory and textural attributes as of conventional meat, consumer acceptance, and proper set up of regulatory framework are challenges faced in commercialization and consumer acceptance of in-vitro meat. To realize any perceivable change in various socio-economic and environmental spheres, the technology should be commercialized and should be cost-effective as conventional meat and widely accepted among consumers. The new challenges of increasing demand of meat with the increasing population could be fulfill by the establishment of in-vitro meat production at large scale and its popularization. The adoption of in-vitro meat production at an industrial scale will lead to self-sufficiency in the developed world.

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          Most cited references156

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          3D bioprinting of tissues and organs.

          Additive manufacturing, otherwise known as three-dimensional (3D) printing, is driving major innovations in many areas, such as engineering, manufacturing, art, education and medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional living tissues. 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation. Compared with non-biological printing, 3D bioprinting involves additional complexities, such as the choice of materials, cell types, growth and differentiation factors, and technical challenges related to the sensitivities of living cells and the construction of tissues. Addressing these complexities requires the integration of technologies from the fields of engineering, biomaterials science, cell biology, physics and medicine. 3D bioprinting has already been used for the generation and transplantation of several tissues, including multilayered skin, bone, vascular grafts, tracheal splints, heart tissue and cartilaginous structures. Other applications include developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
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            Options for keeping the food system within environmental limits

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              3D bioprinting for engineering complex tissues.

              Bioprinting is a 3D fabrication technology used to precisely dispense cell-laden biomaterials for the construction of complex 3D functional living tissues or artificial organs. While still in its early stages, bioprinting strategies have demonstrated their potential use in regenerative medicine to generate a variety of transplantable tissues, including skin, cartilage, and bone. However, current bioprinting approaches still have technical challenges in terms of high-resolution cell deposition, controlled cell distributions, vascularization, and innervation within complex 3D tissues. While no one-size-fits-all approach to bioprinting has emerged, it remains an on-demand, versatile fabrication technique that may address the growing organ shortage as well as provide a high-throughput method for cell patterning at the micrometer scale for broad biomedical engineering applications. In this review, we introduce the basic principles, materials, integration strategies and applications of bioprinting. We also discuss the recent developments, current challenges and future prospects of 3D bioprinting for engineering complex tissues. Combined with recent advances in human pluripotent stem cell technologies, 3D-bioprinted tissue models could serve as an enabling platform for high-throughput predictive drug screening and more effective regenerative therapies.
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                Author and article information

                Journal
                J Anim Sci Technol
                J Anim Sci Technol
                J Anim Sci Technol
                jast
                Journal of Animal Science and Technology
                Korean Society of Animal Sciences and Technology
                2672-0191
                2055-0391
                July 2021
                31 July 2021
                : 63
                : 4
                : 693-724
                Affiliations
                [1 ]Department of Livestock Products Technology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana Punjab 141004, India
                [2 ]Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia , Serdang 43400, Malaysia
                [3 ]Division of Veterinary Medicine, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu , R.S. Pura, UT of Jammu and Kashmir 181102, India
                [4 ]Department of Livestock Production and Management, College of Veterinary Sciences & Animal Husbandry, Nanaji Deshmukh Veterinary Science University , Mhow, Madhya Pradesh 453446, India
                [5 ]Department of Livestock Products Technology, College of Veterinary and Animal Science, Sardar Vallabhbhai Patel University of Agriculture and Technology , Meerut, Uttar Pradesh 250110, India
                [6 ]Natural Medicines and Product Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia , Serdang 43400, Malaysia
                Author notes
                [* ]Corresponding author: Neelesh Sharma, Division of Veterinary Medicine, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, R.S. Pura, UT of Jammu and Kashmir 181102, India. Tel: +91-1923-252906, E-mail: drneelesh_sharma@ 123456yahoo.co.in
                Author information
                https://orcid.org/0000-0002-0567-0359
                https://orcid.org/0000-0003-3932-4064
                https://orcid.org/0000-0001-6530-2155
                https://orcid.org/0000-0002-8856-9490
                https://orcid.org/0000-0002-3741-638X
                https://orcid.org/0000-0003-3169-700X
                https://orcid.org/0000-0002-7362-0855
                Article
                jast-63-4-693
                10.5187/jast.2021.e85
                8367411
                34447949
                ccb202ca-7a1b-4b31-aee2-4649efc5f133
                © Copyright 2021 Korean Society of Animal Science and Technology

                This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 02 June 2021
                : 21 June 2021
                : 22 June 2021
                Funding
                Funded by: Department of Biotechnology, New Delhi;
                Award ID: BT/PR26666/AAQ/1/713/2017
                Categories
                Review
                Custom metadata
                2021-08-31

                cell culture,sustainability,food neophobia,consumer acceptance,future prospects

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